Product Description

Catalog number: E-EL****6
Size: *6T
Reactivity: Universal
Detection Range: 1.*6~**0 μg/mL
Sensitivity: 0.*4 μg/mL
Lead time: 7~*0days 

Intended use
This ELISA kit can be applied to the in vitro quantitative determination of  GSH concentrations in serum, plasma and other biological fluids.

Test principle
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with GSH. During the reaction,  GSH in the sample or standard competes with a fixed amount of GSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  GSH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of **0 nm ± 2 nm. The concentration of  GSH in the samples is then determined by comparing the OD of the samples to the standard curve.