Product Description
INTENDED
USE
ThisB.GP*1 ELISA kit is intended Laboratory
for Research use only and is not for use in diagnostic or
therapeutic procedures. The Stop Solution changes the color from
blue to yellow and the intensity of the color is measured at **0nm
using a spectrophotometer. In order to measure the concentration of
P*1 in the sample, this P*1 ELISA Kit includes a set of calibration
standards. The calibration standards are assayed at the same time
as the samples and allow the operator to produce a standard curve
of Optical Density versus P*1 concentration. The concentration of
P*1 in the samples is then determined by comparing the O.D. of the
samples to the standard curve.
PRINCIPLE OF THE
ASSAY
The coated well immunoenzymatic
assay for the quantitative measurement of serum P*1 utilizes a
monoclonal anti-P*1 and a P**-HRP conjugate. The assay sample and
buffer are incubated together with anti-P*1 antibody coated plate
for sixty and washed. The diluted P**-HRP conjugate is then added
to each well and incubated. After the incubation period, the wells
are decanted and washed three times. The wells are then incubated
with a substrate for the enzyme. The product of the
enzyme-substrate reaction forms a blue colored complex. Finally, a
stopping solution is added to stop the reaction, which will then
turn the solution yellow. The intensity of color is measured
spectrophotometrically at **0nm in a microplate reader. The
intensity of the color is inversely proportional to the P*1
concentration since P*1 from samples and P**-HRP conjugate compete
for the anti-P*1 antibody binding site. Since the number of sites
is limited, as more sites are occupied by P*1 from the sample,
fewer sites are left to bind P**-HRPconjugate. Standards of known P*1
concentrations are run concurrently with the samples being assayed
and a standard curve is plotted relating the intensity of the color
(Optical Density) to the concentration of P*1. The unknown
P*1
concentration in each sample is
interpolated from this curve.
REAGENTS
PROVIDED
All reagents provided are stored at
**8° C. Refer to the expiration date on the label.
1. MICROTITER
PLATE *6 wells
2. ENZYMECONJUGATE 6.0
mL 1 vial
3. STANDARD.1
0ng/ml 1 vial
4. STANDARD.2
0.5ng/ml 1 vial
5. STANDARD.3
1.0ng/ml 1 vial
6. STANDARD.4 2.5ng/ml 1
vial
7. STANDARD.5 5.0ng/ml 1
vial
8. STANDARD.6 *0ng/ml 1
vial
9. SUBSTRATE
A 6.0 mL 1 vial
*0. SUBSTRATE
B 6.0
mL 1 vial
*1. STOP
SOLUTION 6.0
mL 1 vial
*2. WASH
SOLUTION x**0 *0 mL 1 vial
*3. Instruction
1
SAMPLE COLLECTION AND
STORAGE
Serum-Use a serum separator tube(SST) and
allow samples to clot for *0minutes before centrifugation for
*5minutes at approximately ***0xg. Remove serum and assay
immediately or aliquot and store samples at **0 °C or
**0°C.
Plasma- Collect plasma using EDTA or
heparin as an anticoagulant. Centrifuge samples for *5 minutes at
***0 x g at **8°C within *0minutes of collection. Store samples at
**0°C or **0°C.Avoid repeated freeze-thaw cycles.
Cell culture fluid and other
biological fluids- Remove particulates by
centrifugation and assay immediately or aliquot and store samples
at **0°C or **0°C.Avoid repeated freeze-thaw cycles.
NOTE:Serum, plasma, and cell culture
fluid samples to be used within 7 days may be stored at **8°C,
otherwise samples must stored at **0°C(≤2months) or
**0°C(≤6months)
to avoid loss of bioactivity and contamination. Avoid freeze-thaw
cycles .When performing the assay slowly bring samples to room
temperature.
DO NOT USE HEAT-TREATED
SPECIMENS.
MATERIALS REQUIRED
BUT NOT SUPPLIED
1. Microplate
reader capable of measuring absorbance at **0 nm.
2. Precision
pipettes to deliver 2 ml to 1 ml volumes.
3. Adjustable
*0ml ***0ml pipettes for reagent preparation.
4. Adjustable
*0ml ***0ml pipettes for reagent preparation.
5. **0
ml and 1 liter graduated cylinders.
6. Calibrated
adjustable precision pipettes, preferably with disposable plastic
tips. (A manifold multi-channel pipette is desirable for large
assays.)
7. Absorbent
paper.
8. *7°C
incubator.
9. Distilled
or deionized water.
*0. Data
analysis and graphing software. Graph paper: linear (Cartesian),
log-log or semi-log, or log-logit as desired.
*1. Tubes
to prepare standard or sample dilutions.
PRECAUTIONS
1. Do
not substitute reagents from one kit lot to another. Standard,
conjugate and microtiter plates are matched for optimal
performance. Use only the reagents supplied by
manufacturer.
2. Allow
kit reagents and materials to reach room temperature (****5°C)
before use. Do not use water baths to thaw samples or
reagents.
3. Do
not use kit components beyond their expiration date.
4. Use
only deionized or distilled water to dilute reagents.
5. Do
not remove microtiter plate from the storage bag until needed.
Unused strips should be stored at **8°C in their pouch with the
desiccant provided.
6. Use
fresh disposable pipette tips for each transfer to avoid
contamination.
7. Do
not mix acid and sodium hypochlorite solutions.
8. Serum
and plasma should be handled as potentially hazardous and capable
of transmitting disease. Disposable gloves must be worn during the
assay procedure, since no known test method can offer complete
assurance that products derived from human blood will not transmit
infectious agents. Therefore, all blood derivatives should be
considered potentially infectious and good laboratory practices
should be followed.
9. All
samples should be disposed of in a manner that will inactivate
viruses.
*0. Solid
Waste: Autoclave *0 min. at **1°C.
*1. Liquid
Waste: Add sodium hypochlorite to a final concentration of 1.0%.
The waste should be allowed to stand for a minimum of *0 minutes to
inactivate the viruses before disposal.
*2. Substrate
Solution is easily contaminated. If bluish prior to
use,
| Country: |
China |
| Model No: |
E01A0004
|
| FOB Price: |
(Negotiable)
Get Latest Price
|
| Place of Origin: |
- |
| Price for Minimum Order: |
- |
| Minimum Order Quantity: |
- |
| Packaging Detail: |
- |
| Delivery Time: |
- |
| Supplying Ability: |
- |
| Payment Type: |
- |
| Product Group : |
bluegene
|
