Product Description

Cat. No.  DM**1

Ordering information

Storage: at**0oC for two years

Description

EasySee®Western Marker is composed of eight proteins ranging from *0 kDa to *0 kDa. Protein bands of *0 kDa, *5 kDa and *5 kDa are prestained allowing easy identification and monitoring of your proteins during electrophoresis and membrane transfer. Other five proteins contain several IgG binding sites, allowing marker visualization using the same reagents and protocol for your target proteins. These no-dye-detached proteins result in more accurate molecular weight estimation.

• Prestained bands for monitoring electrophoresis and membrane transfer.

• Visualizing molecular weight marker on western blots with the commonly used immuno-detection methods.

• Ready-to-use format, no heating and reductant are required.

Advantages

• When using chemiluminescent detection by alkaline phosphatase- or horseradish peroxidase-based system, the protein molecular weight standard bands (*5 kDa, *0 kDa, *0 kDa, *5 kDa and *0 kDa) are displayed.

• Accurate identification of protein molecular weight, without need of labeling and comparison with protein standard bands.

• EasySee® Western Marker provides accurate protein molecular weight, due to no label and no dye detached.

• Ready-to-use format, no heating and reductant agents are required.

Storage buffer

**5 mM Tris-HCl (pH 6.8), 5 mM EDTA, *0 mM DTT, *7.4% glycerol, 3% SDS, 0.*1% phenol red

Instruction for Use

• It is in a ready-to-use format without heating and reducing agent.

• Completely thaw and mix well before use and use the following volumes of the ladder on *0% Tris-glycine SDS gel: 5 μl per well for mini gel; *0 μl per well for large gel.

Electrophoresis condition

BioRad Mini Electrophoresis installation and transfer system. Electrophoresis at **0 V for *0 minutes, transfer to membrane at **0 mA for **3 hours.

CITATIONS

Zhang YM. et al. ***9. Generate Western blot protein marker from a single construct. **0(2):****8. Anal Biochem. IF= 2.**6.  PMID: *******7.

Kao CH. et al. ***2. A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis. **1(1):**3. Anal Biochem. IF= 2.**6.  PMID: *******2.

Liu H. et al. ***9. The mechanism involved in the repression of the mu opioid receptor gene expression in CEM x**4 cells infected by simian immunodeficiency virus. *5(4):*****1. J Leukoc Biol. IF= 4.**2.  PMID: *******6.

Shao Q. et al. ***2. Hindgut innate immunity and regulation of fecal microbiota through melanization in insects. **7(*7):******9. J Biol Chem. IF=4.**3.  PMID: *******3.