Product Description

*6Tests
Store all reagents at **8℃
Validity Perid: six months
Drug Names
Generic Name：Fish Follicle-stimulating hormone(FSH) ELISA Kit
Purpose
Our Fish Follicle-stimulating hormone(FSH) ELISA kit is to assay FSH levels in Fish
serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided
in this kit has been pre-coated with an antibody specific to FSH. Standards or samples are
added to the appropriate Microelisa stripplate wells and combined to the specific antibody.
Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for FSH is added to each
Microelisa stripplate well and incubated. Free components are washed away. The TMB
substrate solution is added to each well. Only those wells that contain FSH and HRP
conjugated FSH antibody will appear blue in color and then turn yellow after the addition of
the stop solution. The optical density (OD) is measured spectrophotometrically at a
wavelength of **0 nm. The OD value is proportional to the concentration of FSH. You can
calculate the concentration of FSH in the samples by comparing the OD of the samples to the
standard curve.