Product Description

HS-Fuzz DNA Polymerase is a unique artificial enzyme created on the basis of intellectual protein design planning by genetic engineering technique.
HS-Fuzz emzymatic activity at ambient temperature is blocked by highly specific monoclonal antibodies against HF-Fuzz polymerase. Antibodies allows to switch enzymatic activity during first denaturation step of PCR run, but keeps HF-Fuzz inactive during PCR reaction setup.
This feature results in improovement of specificity of PCR and increasing of yeild of PCR product, preventing miss priming and PCR artifacts formation – primer-dimers.

The enzyme possess high fidelity feature. The processivity of the enzyme is very high, so the combination of processivity with fidelity results in dramatically increased yield of PCR products, very high sensitivity of PCR tests, ability to amplify “difficult” templates.This dramatic increase in processivity results not only in shorter extension times, but in more robust amplification and the ability to amplify long templates really fast.

HS-Fuzz DNA Polymerase possesses the 5'->3' DNA polymerase activity, 3'->5' exonuclease activity and temperature-depended strand-displacement activity and generates blunt ends in the amplification products.

HS-FuzzProvides:

- Superior fidelity - *0x improvement compared to Taq polymerase;

- Excellent performance across a wide range of “difficult” templates;

- Long range amplification of complex targets - > *0 kb from genomic DNA;

- High speed - reduce reaction times.

- dUTP poisoning resistance

- Resistance to blood containing DNA samples

(up to *0% of blood)