Product Description

In biological experiments, we should often use biological buffer, and in biological buffer, Tris is commonly used. As a widely used biological buffer, many people should know the name, but some people still don't know it. Let's take a look at the principle and application of Tris.

Tris, also known as trimethylol aminomethane, is a white crystalline powder with CAS number 77-86-1. Soluble in ethanol and water, but also with ethyl acetate, but relatively slow, not with ether, carbon tetrachloride, copper, aluminum corrosion.

Tris is widely used in acute metabolic and respiratory acidemia, which is an alkaline buffer. It has a good buffer effect on metabolic acidosis and enzyme activity reaction, and may cause hypoglycemia, hypotension, nausea and vomiting. It can even inhibit breathing and stop breathing. Excessive intake or renal insufficiency can cause alkalescence. When Tris and acid titration reference materials are used, the purity requirement will be very high, basically 99.9% - 100%

Because Tris is weakly alkaline, PKA is 8.1 when the temperature is below 25 ℃, but according to the buffer theory, its effective buffer range is between ph7.0-9.2. The pH value of Tris aqueous solution is about 10.5, and hydrochloric acid is usually added to adjust the pH value to the desired value, but the effect of temperature on the pKa of Tris should also be noted. Because Tris buffer is a weak alkaline solution, DNA will be deprotonated in the solution, thus improving its solubility. Therefore, someone often adds EDTA to Tris hydrochloric acid buffer to make te buffer, and changes the solution adjusted by pH value to acetic acid to obtain Tae buffer.

Desheng biochemical is a big producer of trimethylol aminomethane in China. You can contact us if you need. Not only the product quality is good, but also the price is very favorable!