Description

LH ELISA KiT Description:

Materials Provided with LH ELISA KIT:
1.Antibody-coated microtiter wells, *6 wells per bag.

Materials Required, not Provided:
1.Precision pipettes: 0.*4~0.2mL and 1.0 mL.

LH ELISA KiT Background Information:
Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing
hormone-releasing hormone (LH-RH or Gn-RH), which is released by the hypothalamus. LH, also called interstitial cellsti-mulating hormone (ICSH) in men, is glycoprotein with a molecular weight of approximately *0,**0 daltons. It is composed of two noncovalently associated dissimilar amino acid chains, alpha and beta. The alpha chain is similar to that found in human thyroid-stimulating hormone (TSH), folliclestimulating hormone (FSH), and human chorionic gonadotropin(hCG). The differences between these hormones lie in the amino acid composition of their beta subunits, which account for their immunological differentiation. In the differential diagnosis of hypothalamic, pituitary, or gonadal dysfunction, assays of LH concentration are routinely performed in conjugation with FSH assays since their roles are closely interrelated. Furthermore, the hormone levels are used to determine menopause, pinpoint ovulation, and monitor endocrine therapy.

LH ELISA KIT Principle:

The LH Quantitative Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one anti-LH antibody for solid phase (microtiter wells) immobilization and another mouse monoclonal anti-LH antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the antibodies, resulting in the LH molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a *0 minute incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB is added and incubated for *0 minutes, resulting in the development of a blue color. The color development is stopped with the addition of 2N HCl, and the color is changed to yellow and measured spectrophotometrically at **0 nm. The concentration of LH is directly proportional to the color intensity of the test sample.

Notable Features of LH ELISA KiT:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results