Product Description

ELISA test kits on Animal disease detection, food safety test, etc.
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Aflatoxin B1 ELISA Kit（*****1）
1 Introduction
Aflatoxins are highly toxic and carcinogenic metabolites produced by a variety of molds, mainly present in
the cereals, nuts, cottonseed and some products related to animal feeds. Aflatoxin B1 (AFB1 for short) is
one kind of Aflatoxins, whose toxicity, carcinogenicity, contamination frequency are at the top. TLC has
been a commonly used method to detect Aflatoxin, but the sample preparation and analysis is
time-consuming and laborious. While AFB1 ELISA Kit can quickly and accurately analyze Aflatoxin B1
residues of samples.
Packing unit: *6T (including standards).
Test time: *0 minutes
2 Application
The kit is applied to the quantitative detection of Aflatoxin B1 in feed, cereals, edible oil, bread and
other samples.
3 Test principle
The Aflatoxin B1 in samples competitively bind specific antibodies against the Aflatoxin B1 antigen
coated onto polystyrene microwells. Secondary antibodies labeled enzyme can react with the antibody.
The addition of an enzyme substrate develops a blue colour in the presence of enzyme. A stop solution is
added which changes the chromogen colour from blue to yellow. The microwells are measured optically by
a microplate reader with an absorbance filter of **0nm. The intensity of the colour is inversely proportional
to the amount of Aflatoxin B1 in the standard or sample and the amount of Aflatoxin B1 in sample is
calculable.
4 Kit content
4.1 1×Microwells plate (1×*6wells)
4.2 1×Aflatoxin B1 enzyme-conjugate (6mL)
4.3 1×Aflatoxin B1 Ab-Solution (6ml)
4.4 1×Concentrated Sample diluent *0×(*0 ml)
4.5 1×Concentrated Washing buffer *0× (*0ml)
4.6 1×Substrate Solution (*2ml)
4.7 1×Stop solution: 1×bottle (7ml)
4.8 6×Aflatoxin B1 standards: 1ml/each (0ng/mL，0.*5ng/mL，0.*5ng/mL，0.*5ng/mL，1.*5ng/mL，
4.*5ng/mL)



Beijing Yisen biotechnology co., Ltd., located in Beijing light industrial park , China,
 is an emerging high-technology company specialized in the research and
development of: Rapid test kits (Colloidal gold), PCR and ELISA test kits on Animal disease detection, food safety and human disease test, etc.
 
With years of efforts, we keep increasing our products criteria and now all products quality and performance is very steady. They have advantages of fast, simple, high sensitivity, high specificity, 
simple sample preparation, and rapid analysis. 
The products cover genetically modified organism (GMO) test,
 allergens test, species identification, microbiology test, microbial and animal disease test
 as well as the inspection and quarantine. 
 
With the highest level and most advanced *****-level clean operating plant, advanced testing equipment and professional R&D team. Yisen
 provides customers with comprehensive and high cost performance testing products and technical services. 
 
At present, Yisen has got national approval of number of invention patents on 
food safety and animal disease.