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Human Complement Component 2 (C2) Elisa Kit

Human Complement Component 2 (C2) Elisa Kit

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China

Model No:

E01C0360

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shanghai bluegene biotech company

China

Free Member

Contact Person tang

hongliu, Shanghai, Shanghai

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Product Specification

Product Description

INTENDED USE

ThisB.G C2 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at **0 nm using a spectrophotometer. In order to measure the concentration of C2 in the sample, this C2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus C2 concentration. The concentration of C2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PRINCIPLE OF THE ASSAY

This C2 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for C2. Standards or samples are then added to the microtiter plate wells and C2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C2 are added to each well to “sandwich” the C2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain C2 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of **0 nm.

REAGENTS PROVIDED

All reagents provided are stored at **8° C. Refer to the expiration date on the label.

1	MICROTITER PLATE	*6 wells
2	ENZYMECONJUGATE	*0.0 mL	1 vial
3	STANDARD.1	0 ng/mL	1 vial
4	STANDARD.2	1.0ng/mL	1 vial
5	STANDARD.3	2.5ng/mL	1 vial
6	STANDARD.4	5.0ng/mL	1 vial
7	STANDARD.5	*0ng/mL	1 vial
8	STANDARD.6	*5ng/mL	1 vial
9	SUBSTRATE A	6.0 mL	1 vial
*0	SUBSTRATE B	6.0 mL	1 vial
*1	STOP SOLUTION	6.0 mL	1 vial
*2	WASH SOLUTION x**0	*0 mL

Country:	China
Model No:	E01C0360
FOB Price:	(Negotiable) Get Latest Price
Place of Origin:	-
Price for Minimum Order:	-
Minimum Order Quantity:	-
Packaging Detail:	-
Delivery Time:	-
Supplying Ability:	-
Payment Type:	-
Product Group :	-