Product Description

Accuvis’s Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and genomic DNA simultaneously from a single sample of plants. The total RNA and genomic DNA are both column purified in under *0 minutes using a single column. It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. Traditionally the RNA and DNA would be isolated from different aliquots of a sample, however this novel technology will allow for their simultaneous isolation from the same sample. This will not only save time, but will also be of a great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results.

Purification is based on spin column chromatography using Accuvis’s proprietary resin as the separation matrix.  Briefly, the cells or tissue of interest are lysed  with the provided Lysis Solution, ethanol is then added to the lysate, and the solution is loaded onto a spin-column (BIND).  Under these conditions only the RNA and DNA will bind to the column while the proteins are removed in the flowthrough.  Next, an optional step can be carried out in which the genomic DNA can be digested allowing for a more pure RNA sample to be isolated.  Alternatively, traces of the RNA may be digested at this point if a pure sample of genomic DNA is required instead.   The bound nucleic acids are then washed in order to remove any remaining impurities (WASH).   Lastly, the purified nucleic acids are eluted into the provided Elution Buffer or water (ELUTE). Please see the procedure flowchart to the right.

• Complete column purification - The RNA and DNA are both column purified simultaneously using the same column.
• Reduce variability - RNA and DNA are isolated from a single plant sample with no splitting of the lysate, thus reducing inconsistent results and variability.
• Isolate from small samples - Simultaneous isolation of RNA and DNA from a single sample. Ideal for precious, difficult to obtain or small samples.
• Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA, without the use of phenol or chloroform.
• Isolate DNA-free plant RNA or RNA-free plant DNA - Optional protocols for on-column DNase or RNase digestion are provided if the user wishes to isolate pure, DNA-free RNA or pure, RNA-free DNA.