Product Description
Accuvis’s Total RNA Purification Kit provides a rapid method for
the isolation and purification of total RNA in as little as *0
minutes. The kit purifies all sizes of RNA, from large mRNA and
ribosomal RNA down to microRNA (miRNA) and small interfering RNA
(siRNA), without the use of phenol or chloroform. Accuvis's
kit is the only kit on the market that isolates true
total RNA, as other kits must use phenol to recover all sizes of
RNA. Therefore Accuvis's kit offers significant
advantages in functionality, savings on
cost, ease-of-use, no hazardous organic waste, and
no inhibitory effect on PCR amplifications as a result of
residual phenol. With this kit both miRNA and mRNA can be
studied from the same sample without further purifications,
thus offering considerable advantages when comparing and
relating expression of miRNA to other RNA. Furthermore, this is an
excellent kit for the extraction of miRNA from all samples
including plasma.
Total RNA can be isolated from a broad range of sample sources with
this kit including cultured cells, tissues, blood, serum,
plasma, bacteria, yeast, fungi, plants and viruses. Accuvis's
Total Purification RNA Kit works very well with small sample s
and does not require carrier RNA for the extraction. Total
RNA can be isolated from as little as a single cell using this
kit.
Purification is based on spin column chromatography using Accuvis’s
proprietary resin as the separation matrix. Briefly, the cells or
tissue of interest are first lysed using the provided Lysis
Solution, ethanol is added and the RNA is bound to
Accuvis's column (BIND). Under
these conditions only the RNA will bind to Norgen's resin while
most of the contaminating cellular proteins are removed in the
flowthrough or retained on top of the resin. The
bound RNA is then washed to remove any remaining
impurities (WASH). Lastly,
the purified total RNA is eluted into *0 µL of the provided Elution
Buffer or water(ELUTE). Please
see procedure flowchart to the right.
Norgen’s proprietary resin provides superior affinity to the full
size range of RNA molecules, resulting in large and small RNA
(miRNA) purification with better linearity and sensitivity. The
purified RNA is of the highest integrity, and can be used in a
number of downstream applications including real time
RT-PCR, RT-PCR, Northern blotting, RNase protection and primer
extension, expression profiling, miRNA cloning and
amplification and Next Generation Sequencing.
- No phenol:chloroform extractions - Total
RNA is isolated without the use of harmful chemicals such as phenol
or chloroform
- Isolate total RNA from very small samples
- Total RNA has been isolated and detected from as
little as a single animal cell
- Extremely efficient isolation of low abundance microRNA
- Accuvis's Total RNA Purification Kit has been
shown to be extremely efficient at recovering low abundance
microRNA from plasma samples (link)
- Isolate a diversity of RNA species - All
RNA species can be isolated, from large mRNA and ribosomal RNA down
to microRNA (miRNA) and small interfering RNA (siRNA)
- Fast and easy processing - Rapid
spin-column format allows for the processing of *0 samples in *0
minutes
- Isolate total RNA from a broad source
- Total RNA has been isolated from cultured animal
cells, small tissue samples, LCM samples, bacteria, yeast,
fungi, plants, viruses and various bodily fluid including blood,
plasma, serum, saliva, nasal or throat swabs
- No need for carrier RNA - Isolate all
sizes of RNA without the use of carrier RNA
- Multiple kit sizes available - This kit
is available in both *0 prep and **0 prep sizes
| Country: |
UAE |
| Model No: |
AV1005
|
| FOB Price: |
(Negotiable)
Get Latest Price
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| Place of Origin: |
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| Price for Minimum Order: |
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| Minimum Order Quantity: |
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| Packaging Detail: |
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| Delivery Time: |
10-12 days |
| Supplying Ability: |
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| Payment Type: |
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| Product Group : |
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