Product Description
Description
Human Keratinocyte
growth factor*2 (KGF*2 or FGF**0) is a novel human protein that
stimulates the repair of injured mucosal tissues. KGF*2 directly
promotes wound-healing by stimulating the proliferation, migration
and differentiation of epithelial cells, and has direct chemotactic
effects on tissue remodeling. KGF*2 also appears to result in less
scarring.
Recombinant Human KGF*2
produced in E.Coli is a single, non-glycosylated polypeptide chain
containing **0 amino acids with molecular weight of about *9.3kD.
Recombinant human KGF*2 is highly related to KGF*1(FGF*7) and binds
to the same receptor as KGF*1 and shares *7% sequence homology.
Recombinant KGF*2 is purified by proprietary chromatographic
techniques.
Source:Escherichia
Coli.
Physical
Appearance:Sterile
Filtered White lyophilized (freeze-dried)
powder.
Formulation:Lyophilized
from a concentrated (1mg/ml) solution in water containing *0mM
Sodium Phosphate buffer, pH 7.4 , *0 mM
NaCl.
Solubility:It
is recommended to reconstitute the lyophilized KGF *2 in sterile
*8MΩ-cm H2O not less than **0ug/ml, which can
then be further diluted to other aqueous
solutions.
Stability:Lyophilized
KGF*2 although stable at room temperature for 3 weeks, should be
stored desiccated below **8℃ . Upon reconstitution KGF*2 should be
stored at 4℃ between **7 days and for future use below **8℃ . For
long term storage it is recommended to add a carrier protein (0.1%
HSA or BSA).
Please avoid freeze-thaw
cycles.
Amino-Acid Sequence
:The sequence of the first five N-terminal amino
acids was determined and was found to be
Met-Ala-Leu-Gly-Asp.
Dimers and aggregates
:Less than 1% as determined by silver-stained
SDS-PAGE gel analysis.
Biological Activity
:Recombinant human KGF*2 is fully biologically
active when compared to standards. The specific activity is about
1.0 x *0 6 Units/mg.
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China |
Model No: |
005
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